ABSTRACT
Objective To investigate the effect of different explants on emodin contents in the callus of Polygouum cuspidatum by reverse-phase HPLC. Methods The leafs,stems and leaf stalks of Polygouum cuspidatum were used as explants and induce the callus by MS medium containing 6-BA 0.2 mg/L,KT 0.2 mg/Land 2,4-D 0.5 mg/L. The contents of emodin were analyzed by HPLC. The separation was performed on Diamonsil-C18 (250 mm?4.6 mm,5 ?m) column,using methanol-0.1% H3PO4 (80∶20) as mobile phase,detected at 254 nm. The flow rate was at 1.0 mL/min,the column temperature was 27 ℃. Results The contents of emodin were 0.709 6,1.680 0 and 0.166 8 mg/g in the callus induced by leafs,stems and leaf stalks. The linear range of emodin was 0.25～5 ?g/mL,r=0.999 85,the average recovery was 101.19% and RSD was 1.20%. Conclusion HPLC method could be used for the contents determination of emodin in Polygouum cuspidatum callus and the contents of emodin was high in the callus induced by stems.
ABSTRACT
Objective To establish a HPLC method for the determination of Ferulic acid in callus of Ligusticum chuanxiong. Methods The separation was performed on ZORBAX 300SB-C18 (250 mm?4.6 mm, 5 ?m) column, using acetonitrile-1% triethylamine (15∶85) as mobile phase, detected at 320 nm. The flow rate was 1.0 mL/min, the column temperature was 27 ℃. Results The linear range of Ferulic acid was 0.93～4.67 ?g/mL, r =0.999 8, the average recovery was 99.98% and RSD was 1.63%. Conclusion The method was rapid, simple, accurate and stability, and can be used for the quantitiative determination of Ferulic acid in Ligusticum chuanxiong callus.